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Effect of ABT-737 on PANoptosis-related protein expression in cultured cells. A, B Western blot analysis and quantification of necroptosis markers (RIPK1, RIPK3, MLKL, pMLKL). C, D Apoptosis markers (caspase-3, cleaved caspase-3, Bax, Bcl-2). E, F Pyroptosis markers (NLRP3, ASC, <t>caspase-1).</t> β-actin was used as loading control. Data are presented as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control
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Effect of ABT-737 on PANoptosis-related protein expression in cultured cells. A, B Western blot analysis and quantification of necroptosis markers (RIPK1, RIPK3, MLKL, pMLKL). C, D Apoptosis markers (caspase-3, cleaved caspase-3, Bax, Bcl-2). E, F Pyroptosis markers (NLRP3, ASC, <t>caspase-1).</t> β-actin was used as loading control. Data are presented as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control
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Effect of ABT-737 on PANoptosis-related protein expression in cultured cells. A, B Western blot analysis and quantification of necroptosis markers (RIPK1, RIPK3, MLKL, pMLKL). C, D Apoptosis markers (caspase-3, cleaved caspase-3, Bax, Bcl-2). E, F Pyroptosis markers (NLRP3, ASC, <t>caspase-1).</t> β-actin was used as loading control. Data are presented as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control
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Effect of ABT-737 on PANoptosis-related protein expression in cultured cells. A, B Western blot analysis and quantification of necroptosis markers (RIPK1, RIPK3, MLKL, pMLKL). C, D Apoptosis markers (caspase-3, cleaved caspase-3, Bax, Bcl-2). E, F Pyroptosis markers (NLRP3, ASC, caspase-1). β-actin was used as loading control. Data are presented as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control

Journal: Discover Oncology

Article Title: ABT-737 efficiently inhibits hepatocellular carcinoma cell activity via regulating PANoptosis

doi: 10.1007/s12672-026-04684-z

Figure Lengend Snippet: Effect of ABT-737 on PANoptosis-related protein expression in cultured cells. A, B Western blot analysis and quantification of necroptosis markers (RIPK1, RIPK3, MLKL, pMLKL). C, D Apoptosis markers (caspase-3, cleaved caspase-3, Bax, Bcl-2). E, F Pyroptosis markers (NLRP3, ASC, caspase-1). β-actin was used as loading control. Data are presented as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control

Article Snippet: The necroptosis inhibitor Necrostatin-1 (Nec-1) and the caspase-1 inhibitor VX-765 were purchased from MedChemExpress (USA).

Techniques: Expressing, Cell Culture, Western Blot, Control